cell lines hmgu1 induced pluripotent stem cells cf ipsc hmgu isfi001 a n2a cells atcc ccl 131 hmgu1 induced pluripotent stem cells map1b wt  (ATCC)

Journal: Drug Delivery

Article Title: Development of a novel alpha7-nicotinic acetylcholine receptor-selective cell-penetrating peptide for intracellular cargo transport

doi: 10.1080/10717544.2025.2587378

Figure Lengend Snippet: RVG, α -btx lp2, and ARA peptides are not cytotoxic at functional concentrations. Cytotoxicity profiles for (A) the RVG parent peptide, (B) the α -btx lp2 peptide, and (C) ARA using α7 nAChR-transfected N2a cells. Peptides (0.03–100 μM) that were preapplied for 24 hour and assessed by the alamarBlue Cell Viability Assay show no significant cytotoxic effects (one-way ANOVA with Tukey's multiple comparison test, **** P < 0.0001). 15% DMSO was the positive control. Points are the mean ± S.D. ( N = 3, n = 6–9).

Article Snippet: The mouse N2a cell line was purchased from ATCC.

Techniques: Functional Assay, Transfection, Viability Assay, Comparison, Positive Control

Journal: Drug Delivery

Article Title: Development of a novel alpha7-nicotinic acetylcholine receptor-selective cell-penetrating peptide for intracellular cargo transport

doi: 10.1080/10717544.2025.2587378

Figure Lengend Snippet: Demonstration of α7 nAChR-specific labeling with α -btx-AF647. Confocal images of (A) α7 nAChR-transfected, (B) nontransfected, and (C) α7 nAChR siRNA-transfected N2a cells treated with 80 nM α -btx-AF746 for 24 hour show the selective binding of α -btx-AF647 to cells overexpressing α7 nAChRs and little binding to cells with residual endogenous expression. (D) Untreated N2a cells were imaged in the AF647 fluorescence channel. Α-btx-AF647 selectively interacts with cells expressing α7 nAChRs and interacts little with cells not transfected with α7 nAChRs. (A’–D’) Same as (A-D) but without the phase channel. Quantification of CTCF (E) demonstrates high fluorescence in cells expressing α7 nAChRs, with no significant fluorescence in non-transfected and α7 nAChR siRNA-transfected cells (one-way ANOVA with Tukey's multiple comparison test, N = 4, n = 120–174). The values are the means ± S.D. and were normalized to those of untreated controls (D).

Article Snippet: The mouse N2a cell line was purchased from ATCC.

Techniques: Labeling, Transfection, Binding Assay, Expressing, Fluorescence, Comparison

Journal: Drug Delivery

Article Title: Development of a novel alpha7-nicotinic acetylcholine receptor-selective cell-penetrating peptide for intracellular cargo transport

doi: 10.1080/10717544.2025.2587378

Figure Lengend Snippet: RVG CPP nonselectively interacts with N2a cells. Confocal images of ( A) α7 nAChR-transfected, (B) non-transfected, and (C) α7 nAChR siRNA-transfected N2a cells treated with 30 μM RVG-FITC for 24 hour showing RVG binding to N2a cells regardless of α7 nAChR presence. (D) Untreated N2a cells imaged in the FITC fluorescence channel. RVG interaction with N2a cells is not dependent on the expression of the α7 nAChR. (A’–D’) Same as (A-D) but without the phase channel. Quantification of CTCF (E) confirmed similar fluorescence levels in cells with and without α7 nAChRs (one-way ANOVA with Tukey's multiple comparison test, N = 4, n = 120–135). The values are the means ± S.D. and were normalized to those of untreated controls (D) .

Article Snippet: The mouse N2a cell line was purchased from ATCC.

Techniques: Transfection, Binding Assay, Fluorescence, Expressing, Comparison

Journal: Drug Delivery

Article Title: Development of a novel alpha7-nicotinic acetylcholine receptor-selective cell-penetrating peptide for intracellular cargo transport

doi: 10.1080/10717544.2025.2587378

Figure Lengend Snippet: ARA preferentially interacts with cells expressing α7 nAChRs. Confocal images of (A) α7 nAChR-transfected, (B) nontransfected, and (C) α7 nAChR siRNA-transfected N2a cells treated with 30 μM ARA-FITC for 24 hour showing high fluorescence levels associated with ARA-FITC in cells expressing α7 nAChRs. (D) Untreated N2a cells were imaged in the FITC fluorescence channel to account for autofluorescence. Endogenously expressed α7 nAChRs in N2a cells contribute to the staining of nontransfected N2a cells, which is reduced by the transfection of α7 siRNA. (A’- D’) Same as (A-D) but without the phase channel. The quantification of CTCF (E) demonstrates significantly more fluorescence in cells expressing α7 nAChRs (one-way ANOVA with Tukey's multiple comparison test, N = 4, n = 117–126). The values are the means ± S.D. and were normalized to those of the untreated control (D).

Article Snippet: The mouse N2a cell line was purchased from ATCC.

Techniques: Expressing, Transfection, Fluorescence, Staining, Comparison, Control

Journal: Drug Delivery

Article Title: Development of a novel alpha7-nicotinic acetylcholine receptor-selective cell-penetrating peptide for intracellular cargo transport

doi: 10.1080/10717544.2025.2587378

Figure Lengend Snippet: ARA transports FITC cargo into α7 nAChR-expressing cells. Representative 3D projections from z-stack confocal images of α7 nAChR-transfected N2a cells (z-stack slice size: 1 μm) treated with (A) 30 μM FITC-tagged ARA peptide or (B) 40 nM α -btx-AF647 for 24 hour prior to imaging. (A’ and B’) Rotated around the y-axis by 40°. ARA appears in the cell interior, while α -btx binds to the cell surface without internalization.

Article Snippet: The mouse N2a cell line was purchased from ATCC.

Techniques: Expressing, Transfection, Imaging